Monoamine Oxidase Assay for Drug Discovery

Monoamine oxidases (MAOs) are located at the outer membranes of mitochondria in numerous tissues including the liver, intestinal mucosa, and nerves. MAOs play a major role in inactivating neurotransmitters¹ . Identifying small molecules that may inhibit MAOs at an early stage in drug discovery is critical in limiting costs associated with these small molecules.

Apredica measures inhibition of MAOs using LC/MS analysis of the product, 4-hydroxyquinoline (4-HQ), formed by MAOs from their substrate kynuramine.

Principle of the Assay

Test agent is incubated in a plate-based assay with a recombinant MAO and kynuramine. 4-HQ is analyzed by LC/MS.

MAOs Available

MAO-A and MAO-B.

Sample Requirements

1-2 mg of solid material or DMSO stock solution.

Assay Modes

• Titration: Eight serial dilutions of test agent are tested in duplicate to determine an IC50.
• Screening: Test agent is tested in duplicate at two concentrations to determine % inhibition.

Customization is routinely provided. Contact us to learn more about Apredica's Monoamine Oxidase Assay.

Footnotes

1. A High-Throughput Monoamine Oxidase Inhibition Assay Using Liquid Chromatography with Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2004;18(8):834-40.